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91.
Primary–secondary school transition encompasses multiple social, academic and environmental changes which can negatively impact children’s emotional well-being. Children with Social, Emotional and Mental Health difficulties (SEMH) are believed to be especially vulnerable during this time. However, the voices of children with SEMH are heavily underrepresented in this field within practice and research. The present case study examined how children with SEMH difficulties within one special school experience primary–secondary school transition and how they are supported, in order to make recommendations to improve this period. The case study was qualitative and longitudinal, conducted over 18-months and methodologies included ethnographic observations, child photo-elicitation focus groups (with 11 Year 6 children) and three adult interviews. Findings demonstrated that over primary–secondary school transition children with SEMH difficulties (a) negotiate significant structural changes in support (often unanticipated) and (b) need to feel a sense of safety and belonging. To manage this effectively, transition provision for children with SEMH difficulties needs to consider their short-term emotional well-being whilst still in primary school, in addition to their long-term well-being looking ahead to secondary school. Greater collaboration and communication across schools and stakeholders can help ensure children receive continuity in standards and support.  相似文献   
92.
针对目前智能手机人工贴屏精度差、效率低、成本高的现状,文章提出了一种基于双目机器视觉的高速高精度自动贴屏解决方案。该方案采用两个CCD相机分别采集手机机身和手机屏幕图像,经图像采集、图像预处理后,快速提取图像边缘信息,准确快速计算XY轴向偏差和R向角度偏差,并由XYR对位平台快速完成对位调整。经测试,该设备定位精度≤±0.005mm,重复定位精度≤±0.005mm,贴屏速度≥300片/小时,屏幕贴合平整度≤±0.005mm,能大幅提升贴屏精度和效率,具备一定的应用和推广价值。  相似文献   
93.
基于Photoshop课程和高职院校学生的特点分析,采用网络教学资源、屏幕录像软件及QQ群相结合的教学模式开展线上教学,旨在探索适宜学生学习的线上教学模式,增加学生的学习自主性和学习乐趣,实现分层化和个性化教学,并对教学效果进行评价分析,证明其可行性和适用性。  相似文献   
94.
Since it was first recognized in bacteria and archaea as a mechanism for innate viral immunity in the early 2010 s,clustered regularly interspaced short palindromic repeats(CRISPR)/CRISPR-associated protein(Cas) has rapidly been developed into a robust, multifunctional genome editing tool with many uses. Following the discovery of the initial CRISPR/Cas-based system, the technology has been advanced to facilitate a multitude of different functions. These include development as a base editor, prime editor, epigenetic editor, and CRISPR interference(CRISPRi) and CRISPR activator(CRISPRa) gene regulators. It can also be used for chromatin and RNA targeting and imaging. Its applications have proved revolutionary across numerous biological fields, especially in biomedical and agricultural improvement. As a diagnostic tool, CRISPR has been developed to aid the detection and screening of both human and plant diseases, and has even been applied during the current coronavirus disease 2019(COVID-19) pandemic. CRISPR/Cas is also being trialed as a new form of gene therapy for treating various human diseases, including cancers, and has aided drug development. In terms of agricultural breeding, precise targeting of biological pathways via CRISPR/Cas has been key to regulating molecular biosynthesis and allowing modification of proteins, starch, oil, and other functional components for crop improvement. Adding to this, CRISPR/Cas has been shown capable of significantly enhancing both plant tolerance to environmental stresses and overall crop yield via the targeting of various agronomically important gene regulators. Looking to the future, increasing the efficiency and precision of CRISPR/Cas delivery systems and limiting off-target activity are two major challenges for wider application of the technology. This review provides an in-depth overview of current CRISPR development, including the advantages and disadvantages of the technology,recent applications, and future considerations.  相似文献   
95.
96.
Nucleic acids in plant tissue lysates can be captured quickly by a cellulose filter paper and prepared for amplification after a quick purification.In this study,a published filter paper strip method was modified by sticking the filter paper on a polyvinyl chloride resin(PVC)sheet.This modified method is named EZ-D,for EASY DNA extraction.Compared with the original cetyl trimethylammonium bromide(CTAB)method,DNA extracted by EZ-D is more efficient in polymerase chain reaction(PCR)amplification due to the more stable performance of the EZ-D stick.The EZ-D method is also faster,easier,and cheaper.PCR analyses showed that DNA extracted from several types of plant tissues by EZ-D was appropriate for specific identification of biological samples.A regular PCR reaction can detect the EZ-D-extracted DNA template at concentration as low as 0.1 ng/μL.Evaluation of the EZ-D showed that DNA extracts could be successfully amplified by PCR reaction for DNA fragments up to 3000 bp in length and up to 80%in GC content.EZ-D was successfully used for DNA extraction from a variety of plant species and plant tissues.Moreover,when EZ-D was combined with the loop-mediated isothermal amplification(LAMP)method,DNA identification of biological samples could be achieved without the need for specialized equipment.As an optimized DNA purification method,EZ-D shows great advantages in application and can be used widely in laboratories where equipment is limited and rapid results are required.  相似文献   
97.
我国当前高校创新创业教育课程存在一定的不足,尽管有研究提出了改进对策或建议,但其提出过程缺少科学系统的分析工具,且所提出的对策措施千校一面,缺乏针对性。造成这种状况的本质原因在于未对课程对象(顾客)需求期望进行清晰准确的识别。质量功能展开(QFD)立足于顾客,能有效将顾客需求转化为产品/服务设计要求。因此,将QFD与高校双创课程设计相结合,提出创新创业课程质量功能展开模型(ICQFDM),通过"顾客需求—教学规划"与"教学规划—教学管理"两级质量屋,将顾客对于双创课程的要求转化到课程设计要求中。以南京已开展创新创业课程且互为竞争对手的2所财经类院校为例,演示模型的具体运用过程。结果显示:2所高校创新创业课程设计路径存在较大差异。ICQFDM的提出为高校建设创新创业课程提供了可操作的分析框架,各高校可结合不同实际情况,制定适用于自身的课程建设措施。  相似文献   
98.
"业绩管理"(F5)课程涵括成本核算与管理、预算编制与控制、管理决策制定以及业绩评价等内容,要求学生具备一定的综合应用能力,需要学生根据不同商业情景选择适当的分析工具,做出正确的评估。但是在校本科学生缺乏实际工作经验,对外部商业环境知之甚少,这就迫切需要教师在该课程教学中引入案例教学,因为案例教学可深化学生对外部商业环境的了解,而且在常规化观点碰撞和交流中,学生的主观能动性和综合应用能力潜力都会得到充分的刺激和发挥。"业绩管理"(F5)课程的特点要求案例选择和设计时要国际化和本土化并举,要具有针对性和层次性,同时还要与课后习题挂钩。好的案例教学模式既有利于学生发散思维,累积能力,也有助于教师在案例教学准备、实施和总结过程中因材施教,教学相长。  相似文献   
99.
19世纪后期至20世纪初期,欧美出现的教育科学研究运动是教育研究领域一次具有深远意义的运动它的出现与这个时期欧美社会新的发展和教育的深刻变革,以及人们对科学、科学与教育关系的认识有密切的联系。根据欧美教育科学研究运动中一些突出的事件加以划分,其发展可以分为四个阶段,且每个阶段都有其显著的特点。19世纪末至20世纪初期,欧美出现的教育科学研究运动是现代科学研究思想和方法在教育研究领域里的初次运用和实践,其所关注的领域和所争论的问题值得今天反思与借鉴。  相似文献   
100.
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